代表性科研项目 [1] 广东海洋大学科研启动费资助项目:脂肪酶 T6 甲醇耐受性的分子改造及其机理研究,2024-2027,广东海洋大学科,主持 [2] 国家自然科学基金杰出青年基金项目:酶工程,2018- 2022,国家自然科学基金委员会,参与 [3] 广东省促进经济高质量发展专项资金海洋经济发展项目:海洋脂类与糖类转化酶的研制与产业化,2018- 2021,广东省自然资源厅,参与 代表性论文 [1] Lilang Li, Weikun Wu, Zixiao Deng, Shihai Zhang, Wutai Guan. Improved thermostability of lipase Lip2 from Yarrowia lipolytica through disulfide bond design for preparation of medium-long-medium structured lipids. LWT, 2022, 166: 113786. [2] Lilang Li, Xuejing Mao, Fuli Deng, Yonghua Wang, Fanghua Wang. Improving both the thermostability and catalytic efficiency of phospholipase D from Moritella sp. JT01 through disulfide bond engineering strategy. International Journal of Molecular Sciences, 2022, 23(19): 11319. [3] Lilang Li, Yonghua Wang, Ruiguo Cui, Fanghua Wang, Dongming Lan*. Engineering the thermostability of the mono-and diacylglycerol lipase SMG1 for the synthesis of diacylglycerols. Foods, 2022, 11(24): 4069. [4] Lilang Li, Shihai Zhang, Weikun Wu, Wutai, Zixiao Deng, Hanzhen Qiao (2019). Enhancing thermostability of Yarrowia lipolytica lipase 2 through engineering multiple disulfide bonds and mitigating reduced lipase production associated with disulfide bonds. Enzyme and Microbial Technology, 2019, 126: 41-49. [5] Min Tian, Lilang Li, Zhezhe Tian, Hao Zhao, Fang Chen, Wutai Guan, Shihai Zhang. Glyceryl butyrate attenuates enterotoxigenic Escherichia coli-induced intestinal inflammation in piglets by inhibiting the NF-κB/MAPK pathways and modulating the gut microbiota. Food & Function, 2022, 13: 6282–6292. 代表性授权专利(5项) [1] 王永华,李力浪,蓝东明,杨博.一种甘油酯脂肪酶SMG1突变体及其编码基因与应用, ZL202110004120.9,2023.02.28。 [2] 王永华,李力浪,蓝东明,杨博. 甘油酯脂肪酶突变体G28C-P206C及其编码基因与应用, ZL202110004098.8,2022.04.01。 [3] 管武太,李力浪,吴炜坤,邓子潇,张世海. 一种能提高耐热脂肪酶产量的重组工程菌及其构建方法和应用. ZL 01711220171.5,2020.07.03。 [4] 管武太,李力浪,吴炜坤,邓子潇. 一种组合二硫键的耐热突变脂肪酶及其制备方法与应用, ZL201711220203.1,2020.05.15。 [5] 管武太,邓子潇,李力浪,吴炜坤,张世海. 一种耐热突变脂肪酶及制备方法与应用, ZL201711220144.8,2020.05.15。 |